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KMID : 0379119980260020246
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Korean Journal of Mycology
1998 Volume.26 No. 2 p.246 ~ p.255
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Production of Nitric Oxide in Raw 264.7 Macrophages treated with Ganoderan , the β-Glucan of Ganoderma lucidum
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이준우/Lee, June Woo
정훈/한만덕/이은숙/윤경하/김영권/Jeong, Hoon/Han, Man Deuk/Lee, Eun Sook/Yoon, Kyung Ha/Kim, Young Kweon
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Abstract
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Ganoderan (GAN), an immunomodulating β-glucan of G. lucidum, induces potent antitumor immunity in tumor-bearing mice. This study was set up to elucidate the ability of macrophage activation of GANs. GAN-treated Raw 264.7 macrophages showed enhanced production of nitric oxide (NO). The ability of GANs to produce NO was based on differences in chemical composition of GANs obtained from the mycelium on various carbon sources and mycelial fractionation. The highest NO production was observed in CW-AS-WS polysaccharide which was extracted from the mycelial wall. GAN-treated Raw 264.7 cells gave a 2- to 5-fold (24 hr) formation of NO levels compared with those treated with medium only. Partial removal of the protein in the extracellular GAN by TCA treatment did appreciably reduce its capacity to secrete NO. The mixture effect of GAN and LPS increased the nitric oxide secretion from RAW 264.7. The cell proliferation of GAN-treated Raw 264.7 cell lines inhibited as compared with its control. Of the culture supernatant of macrophage activated by GAN, the percentage of cytotoxicity against mouse leukemia L1210 cells was slightly dependent on the amount of NO in the culture supernatants of the activated-macrophages. These results indicate that the β-glucan-related polysaccharides of the higher fungus activate macrophage and release nitric oxide. It also suggests that marine macrophages possess certain receptors for β-anomeric glucans and play a critical role of β-glucan-related tumor killing mechanism.
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